HEWLETT PACKARD ZORBAX Rapid Resolution Reversed-Phase Columns for Peptide Protein Separation Application Note

This article introduces the features of ZORBAX® Rapid Resolution reversed-phase columns, which can reduce analysis time and solvent usage by 40 to 66 percent (depending on column size), while maintaining equivalent resolution to 5-μm particle sized columns. Rapid Resolution columns with 3.5-μm particles and short column lengths (150, 75, and 50 mm) provide the same separating power as 250- and 150-mm columns using 5-μm particles. They have the same physical characteristics (surface area, pore size, and surface coverage) as 5-μm particles, resulting in the same selectivity and retention times. ZORBAX StableBond Rapid Resolution columns, with both 300 Å and 80 Å pore sizes, use a proprietary bonding technology for stable operation at low pH and high temperature, making them ideal for protein and peptide separations.


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Publication date: 02 July, 2012

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